Cover Image

CD34+ stem cells from umbilical cord blood

Carlo Pafumi, Vito Leanza, Antonio Carbonaro, Gianluca Leanza, Alessandra Iemmola, Giusi Abate, Maria Grazia Stracquadanio, Alfio D’Agati
  • Carlo Pafumi
    Gynaecologic and Obstetric Department, University of Catania, Italy | pafumi@unict.it
  • Vito Leanza
    Gynaecologic and Obstetric Department, University of Catania, Italy
  • Antonio Carbonaro
    Gynaecologic and Obstetric Department, University of Catania, Italy
  • Gianluca Leanza
    Gynaecologic and Obstetric Department, University of Catania, Italy
  • Alessandra Iemmola
    Gynaecologic and Obstetric Department, University of Catania, Italy
  • Giusi Abate
    Gynaecologic and Obstetric Department, University of Catania, Italy
  • Maria Grazia Stracquadanio
    Gynaecologic and Obstetric Department, University of Catania, Italy
  • Alfio D’Agati
    Gynaecologic and Obstetric Department, University of Catania, Italy

Abstract

We describe the relation between umbilical cord clamping time and two different enrichment system of CD34+ stem cells from umbilical cord blood with the proliferative ability and bone marrow reconstitution of the stem cells obtained. After an obstetrician performed the cord blood collection, the purification of stem cells was performed either with a combination of monoclonal antibodies (negative selections) using the Stem Sep method, or with a positive cells selection based on their surface CD34 antigens using the Mini Macs system. An excellent recovery of haematopoietic progenitors [Burst Forming Unit Erythroids (BFUE); Colony Forming Unit Granulocytes and Macrophages (CFU-GM); and Colony Forming Unit Granulocytes, Erythroids, Monocytes and Macrophages (CFU-GME)], inversely related to the increase in clamping time, was performed with the Mini Macs system (54% of colonies, with 90% purity). With Stem Sep method, haematopoietic progenitor’s recovery was 35% (with 80% purity). By applying early clamping of umbilical cord blood we obtained a greater number of CD34+ cells and their clonogenic activity was increased with enrichment. This is a useful technique considering that the number of CD34+ stem cells usually contained from a unit of placental blood is enough for the transplant to a child, but not for an adult. Thus, using these methods, we can get a larger number of CD34+ stem cells which reduces the risk of Graft versus Host Disease also in adult patients, producing survival rates similar to those obtained with transplantation of bone marrow from unrelated donors.

Keywords

CD34+ stem cells; umbilical cord blood, recovery, enrichment.

Full Text:

PDF
HTML
Submitted: 2011-09-16 18:09:13
Published: 2011-10-27 19:08:40
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
1057

Views:
PDF
193
HTML
219

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2011 Carlo Pafumi, Vito Leanza, Antonio Carbonaro, Gianluca Leanza, Alessandra Iemmola, Giusi Abate, Maria Grazia Stracquadanio, Alfio D’Agati

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185